Enzymatic procedure for the determination of the blood alcohol concentration. Alcohol is oxidized in presence of the enzyme Alcohol Dehydrogenase (ADH) and the Coenzyms NAD to the acetaldehyde. The developing NADH is determined by UV-photometrie at a wavelenght of 365 nm. The procedure is automizable (autoanalyzer) and with a sample throughput of 1 sample/min the fastest determination procedure. It is used therefore in the clinical emergency diagnostics for the rapid alcohol determination. Together with the gaschromatografic procedurethe it is the base of the forensic blood alcohol determinatation.
The ADH procedure is used today both the forensic blood alcohol determination in mg/100ml (Great Britain) or in continental Europe in parts per thousand (mg/g) (double determination in the serum and following conversion to full blood content by the devision faktor 1.2 during weight-referred calibration and/or 1.236 during volume-referred calibration) and in the hospital emergency diagnostics as serum alcohol content in g/L (single determination).