Abbrevation: EtG. Ethylglucuronid emerges as a metabolic product of ethanol and "activated" Glururonic Acid (Uridine-5'-diphospho-β-glucuronic acid), and was first found in 1967 in human urine. It is only formed in the liver in presence of ethanol as a direct alcohol metabolite in an amount of 0.02 per cent. The possible time of detection in urine with approximately 80-120 hours resp. 24-36 hours in serum is relatively long. The determination of EtG has forensic importance as a marker for alcohol relapses after a withdrawal, and for cases of re-issuance of the driving license. The Cut-off is currently at 0.5 ng/ml for serum and 1.0 ng/ml for urine samples.
Currently, several institutions worked on the detection of EtG in hair specimens as a long-time alcohol marker in order to get informations about the alcohol comsumption of the last months (1 cm hair length respond to one month). The cut-off will be further discussed and is currently set by the most institutes at 25 to 30 pg/mg hair sample.
Quantitative determination of EtG is done by GC/MS or ELISA (hair: LC/MS/MS) as Trimethylsilylether (fragment ions: 160,261,405) with d5-Ethylglucuronide as an internal standard.